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2….  Mass Spectrometer£¨ÖÊÆ×ÒÇ£©£ºÖÊÆ×ÒÇÊÇÒ»¸öÓÃÀ´²âÁ¿µ¥¸ö·Ö×ÓÖÊÁ¿µÄÒÇÆ÷£¬µ«Êµ¼ÊÉÏÖÊÆ×ÒÇÌṩµÄÊÇ·Ö×ÓµÄÖÊÁ¿ÓëµçºÉ±È£¨m/z or m/e£©¡£·ÖÀëºÍ¼ì²â²»Í¬Í¬Î»ËصÄÒÇÆ÷¡£¼´¸ù¾Ý´øµçÁ£×ÓÔÚµç´Å³¡ÖÐÄܹ»Æ«×ªµÄÔ­Àí£¬°´ÎïÖÊÔ­×Ó¡¢·Ö×Ó»ò·Ö×ÓËéƬµÄÖÊÁ¿²îÒì½øÐзÖÀëºÍ¼ì²âÎïÖÊ×é³ÉµÄÒ»ÀàÒÇÆ÷¡£ÖÊÆ×ÒÇ×îÖØÒªµÄÓ¦ÓÃÊÇ·ÖÀëͬλËز¢²â¶¨ËüÃǵÄÔ­×ÓÖÊÁ¿¼°Ïà¶Ô·á¶È¡£

3. Proteome sample holographic preparation£¨µ°°××éÑùÆ·µÄÈ«Ï¢ÖƱ¸£©£º£¨1£©±£³Öµ°°×ÖʵÄÐÅÏ¢£¨2£©ÊÊÓÃÓÚ·ÖÀëºÍ¼ø¶¨·½·¨£¨3£©²»Í¬µÄÑùÆ·£¬²»Í¬µÄÌáÈ¡¡£

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4.Post translational modification(µ°°×ÖÊ·­ÒëºóÐÞÊÎ)

ëÄÁ´ºÏ³ÉµÄ½áÊø£¬²¢²»Ò»¶¨ÒâζמßÓÐÕý³£ÉúÀí¹¦Äܵĵ°°×ÖÊ·Ö×ÓÒѾ­Éú³É¡£ÒÑÖªºÜ¶àµ°°×ÖÊÔÚëÄÁ´ºÏ³Éºó»¹Ðè¾­¹ýÒ»¶¨µÄ¼Ó¹¤£¨processing£©»òÐÞÊΣ¬Óɼ¸ÌõëÄÁ´¹¹³ÉµÄµ°°×ÖʺʹøÓи¨»ùµÄµ°°×ÖÊ£¬Æä¸÷¸öÑǵ¥Î»±ØÐ뻥Ïà¾ÛºÏ²ÅÄܳÉΪÍêÕûµÄµ°°×ÖÊ·Ö×Ó¡£

5.De novo sequencing(´ÓÍ·²âÐò)

δ֪ëÄ  ´ÓÍ·²âÐòΪµ°°×ÖÊ×éÑо¿ÌṩÁËÒ»ÖÖ²»ÓýèÖúÓÚÈκε°°×ÖÊÐòÁÐÊý¾Ý¿âÐÅÏ¢£¬Ö±½Ó½â¶Á´®ÁªÖÊÆ×Êý¾ÝµÄ·½·¨¡£Æä»ù±¾Ëã·¨Ö÷ÒªÓÉ4¸ö²¿·Ö×é³É£ºÖÊÆ×ͼµÄ¹¹½¨¡¢Àë×ÓÀàÐ͵ÄÈ·¶¨¡¢²âÐòËã·¨ÒÔ¼°´ò·ÖËã·¨¡£

6.Tandem mass spectrometry(´®ÁªÖÊÆ×)

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7.Peptide mass fingerprint(ëÄÖ¸ÎÆÆ×)

peptide molecular weight£¨ëÄ·Ö×ÓÁ¿£©  ëÄÖ¸ÎÆͼÆ×£¬PMF£¬peptide mapping fingerprint,µ°°×ø½âºó²úÉú¶à¸öëÄƬ¶Ï£¬È»ºóÀûÓÃÖÊÆ×£¨Í¨³£ÓÃMALDI£­TOF£©²âÁ¿ÕâЩëĶεķÖ×ÓÁ¿£¬È»ºóÉÏÍø½øÐе°°×Æ׿âËÑË÷£¬ÒÔÈ·Èϴ˵°°×ÊǺÎÖÖµ°°×£¬´ËΪPMF¡£ÕâЩëĶξÍÏñµ°°×µÄÖ¸ÎÆÒ»Ñù£¬ÓÐÁËÕâЩëĶΣ¬¾Í¿ÉÒÔÈ·Èϵ°°×ÁË¡£

8. Collision-induced dissociation(CID)(ÅöײÓÕµ¼½âÀë)£ºÀë×Ó¾­¹ýµçÅçÎíÔ´ÔÚ½øÈëÖÊÁ¿¼ì²âÆ÷Ç°£¬Ê©¼ÓÒ»¶¨µÄµçѹ£¬Ê¹Àë×ÓµÄÔ˶¯Ëٶȴó´óÌá¸ß£¬µ±Àë×ÓÓëÖÐÐÔ·Ö×Óײ»÷ʱ£¬·¢ÉúÈçÏ·´Ó¦£ºA++N→(A+)*→B++C++D+

9.Peptide sequence tag ëÄÐòÁбêÇ©£º½«µ°°×ÖʽøÐÐøÇнµ½â ×öµçÅçÎíÖÊÆײúÉú°üÀ¨¶àµçºÉ·åÔÚÄÚµÄëÄÖÊÁ¿Ö¸ÎÆÆ×£¬Ñ¡ÔñÓÐÒ»¶¨·á¶ÈµÄË«µçºÉ·å¾­ÆøÌåÅöײ»î»¯³ØÅöײºó²úÉúËéƬ£¬ÆäÖаüº¬×ŽṹÐÅÏ¢£¬ÓÉÕâЩÐÅÏ¢¿ÉÒÔÍƳöµ°°×ÖʵÄijһëĶεIJ¿·Ö°±»ùËáÐòÁвⶨ³öµÄ²¿·Ö°±»ùËáÐòÁкÍÆäÐòÁÐÁ½¶ÎµÄÖÊÁ¿³ÆΪëÄÐòÁбêÇ©¡£

10. Post-source decay(Ô´ºóË¥±ä)£º·¢ÉúÔÚÀë×ÓÔ´ºóµÄµÚÒ»¸öÎÞ³¡ÇøÓò£¬Ê±¼ä¿ç¶ÈΪ΢Ã룻ÓÉÓÚ·¢ÉúÔÚÎÞ³¡ÇøÓò£¬ËùÒÔ²úÉúµÄ²»Í¬Æ¬¶ÎÀë×ÓºÍĸÀë×Ó±£³ÖͬÑùËٶȣ¬ÓÃÀë×Ó¾µ·´É䣬½«Æ¬¶ÎÀë×ÓºÍĸÀë×Ó·ÖÀ룬°´ÖÊÁ¿´óСÅÅÁпÉÐγÉÀë×ÓÆ×£¬³ÆΪPSDÆ×

11. Phage display technology£¨ÊɾúÌåչʾ¼¼Êõ£©ÔÊÐí´óµÄëĺ͵°°×ÖÊ¿âÔÚ˿״ÊɾúÌå±íÃæչʾ£¬µ¼Ö¶àëĺ͵°°×ÖʵÄÑ¡Ôñ¡£ ÊɾúÌåչʾ¼¼ÊõÊÇÒ»ÖÖ»ùÒò±í´ï²úÎïºÍÇ׺ÍÑ¡ÔñÏà½áºÏµÄ¼¼Êõ£¬ËûÒԸĹ¹µÄÊɾúÌåΪÔØÌ壬°Ñ´ýÑ¡»ùÒòƬ¶Î¶¨Ïò²åÈëÊɾúÌåÍâ¿Çµ°°×ÖÊ»ùÒòÇø£¬Ê¹ÍâÔ´¶àëÄ»òµ°°×Öʱí´ï²¢Õ¹Ê¾ÓÚÊɾúÌå±íÃ棬½ø¶øͨ¹ýÇ׺͸»¼¯·¨±í´ïÓÐÌØÒìëÄ»òµ°°×ÖʵÄÊɾúÌå

12. Two-dimensional Gel ElectrophoresisË«ÏòÄý½ºµçÓ¾£¨2-DE£©£º

¼´Ë«ÏòÄý½ºµçÓ¾£¨2-DE£©£¬¸Ã¼¼ÊõÊǸù¾Ýµ°°×ÖʵĵȵçµãºÍ·Ö×ÓÁ¿µÄ²îÒ죬Á¬Ðø½øÐд¹Ö±·½ÏòµÄÁ½´ÎµçÓ¾¶ø½«´ý²âµ°°×ÖʽøÐзÖÀë¡£µÚÒ»ÏòΪµÈµç¾Û½¹£¨IEF£©µçÓ¾£¬Æä»ù±¾Ô­ÀíÊÇÀûÓõ°°×ÖÊ·Ö×ӵĵȵçµãµÄ²»Í¬½øÐе°°×ÖʵķÖÀë¡£µÚ¶þÏòΪʮ¶þÍé»ùÁòËáÄÆÒ»¾Û±ûÏ©õ£°·Äý½ºµçÓ¾£¨SDS—PAGE£©£¬Æä»ù±¾Ô­ÀíÊÇÀûÓõ°°×ÖÊ·Ö×ÓÁ¿´óСµÄ²»Í¬½øÐе°°×ÖÊ·ÖÀ룬´Ó¶ø°Ñ¸´ÔÓµ°°×ÖÊ»ìºÏÎïÔÚ¶þάƽÃæÉÏ·Ö¿ª¡£¸Ã¼¼ÊõÊÇÄ¿Ç°µ°°×ÖÊ×éѧÑо¿ÖзֱæÂÊ×î¸ß£¬ÐÅÏ¢Á¿×î´óµÄ·ÖÀë¼¼Êõ£¬ÊDZȽϵ°°×ÖÊ×éѧÑо¿ÖÐÒ²ÊDz»¿ÉÈ¡ÉÙµÄÊֶΡ£

13. Structural biology£¨½á¹¹ÉúÎïѧ£©£º ÒÔÉúÎï´ó·Ö×ÓµÄÌض¨¿Õ¼ä½á¹¹¼°½á¹¹µÄÌض¨Ô˶¯ÓëÆäÉúÎïѧ¹¦ÄܵĹØϵΪ»ù´¡£¬²ûÃ÷ÉúÃüÏÖÏóµÄѧ¿Æ¡£Ñо¿ÌØÊâ·Ö×ÓµÄÐÔÖÊÒÔ¼°·Ö×Ó¼äµÄÏ໥×÷Óã¬ÈçĤµ°°×µÄÍØÆËѧ¡¢µ°°×ÖʵĶþ¼¶½á¹¹ÖвлùµÄ½Ó½üºÍÒƶ¯ÒÔ¼°µ°°×ÖʵÄÈý¼¶ÕÛµþµÈ¡£

14. Protein-protein interaction£¨µ°°×ÖÊ»¥×÷£©£º£¨1£©µ°°×¿ÉÄÜ»áÏ໥×÷ÓÃÐγɵĵ°°×Öʸ´ºÏÌ壨2£©Ò»ÖÖµ°°×ÖÊ¿ÉÄÜЯ´øÁíÒ»ÖÖµ°°×ÖÊ£¨3£©µ°°×ÖÊ»áÓëÁíÒ»¸öµ°°×ÖÊÏ໥×÷ÓöøÀ´Ð޸ġ£µ°°×ÖÊÖ®¼äµÄÏ໥×÷Óᣵ°°×ÖÊÖ®¼äµÄÏ໥×÷ÓÃÊǵ°°×ÖÊ·¢»Óµ÷½Ú×÷ÓõÄÖØÒª·½Ê½¡£

15. Yeast Two-Hybrid Assays£¨½Íĸ˫ÔÓ½»·ÖÎö£©£º ½Íĸ˫ÔÓ½»ÏµÍ³ÀûÓÃÔÓ½»»ùÒòͨ¹ý¼¤»î±¨µÀ»ùÒòµÄ±í´ï̽²âµ°°×£­µ°°×µÄÏ໥×÷Óá£Ö÷ÒªÓжþÀàÔØÌå: a º¬DNA -binding domainµÄÔØÌå; b º¬DNA-activating domainµÄÔØÌå¡£ÉÏÊö¶þÀàÔØÌåÔÚ¹¹½¨ÈںϻùÒòʱ£¬ ²âÊÔµ°°×»ùÒòÓë½á¹¹Óò»ùÒò±ØÐëÔÚÔĶÁ¿òÄÚÈںϡ£ÈںϻùÒòÔÚ±¨¸æÖêÖбí´ï£¬ Æä±í´ï²úÎïÖ»Óж¨Î»ÓÚºËÄÚ²ÅÄÜÇý¶¯±¨¸æ»ùÒòµÄת¼¡£

16. Matrix-assisted laser desorption/ionization»ùÖʸ¨Öú¼¤¹â½âÎöµçÀë

¼¼Êõ (MALDI) £º ÊÇÒÔ¼¤¹âÂö³åÕÕÉäÔڹ̶¨ÓÚƽÃæµç¼«Éϵĵ°°×ÖÊ·Ö×Ó£¬¾­Æ½Ðе糡¼ÓËÙ£¬ÔÙÓÉÖÊÆ×ÒǽøÐзÖÎö¡£ÒÔ²¨³¤337nmµÄµªÆø¼¤¹âÂö³å»÷´ò¹Ì¶¨ÓÚ»ùÖÊÉϵĵ°°×ÖÊ·Ö×Ó£¬Ê¹µÃµ°°×ÖÊ´Ó»ùÖÊÉÏÓÎÀë²¢´øÉÏ΢ÈõµÄÕýµçºÉ¡£´ËʱÔÙ¾­ÓɸßÄÜƽÐе糡¼ÓËÙÌá¸ßºËÖʱÈ£¬½øÈëÖÊÆ×ÒǽøÐзÖÎö£¬´Ë·½Ê½ÄÜ·ÖÎö´øµçÁ¿µÍµÄ·Ö×Ó£¬ÒÔ¼°º¬ÓÐÐí¶à²»Í¬ÖÖÀà·Ö×ӵĻìºÏÎï

17. ÑÇϸ°ûµ°°×ÖÊ×éѧ   ÑÇϸ°ûµ°°×ÖÊ×éѧÊÇÒÔÑÇϸ°û½á¹¹ÈçÑÇϸ°ûÇøÊÒ¡¢Ìض¨µ°°×ÖÊ×é·Ö¡¢Ï¸°ûÆ÷µÈËù°üº¬µÄËùÓе°°×ÖÊΪÑо¿¶ÔÏóµÄÒ»¸öµ°°×ÖÊ×éѧµÄ·Ö֧ѧ¿Æ¡£ÑÇϸ°û½á¹¹ÔÚϸ°ûµÄÉú Ãü»î¶¯Öж¼ÓëÌض¨µÄϸ°û¹¦ÄÜÏàÁªÏµ¡£·ÖÀë¡¢¼ø¶¨ÆäÔÚ²»Í¬ÉúÀí״̬ϵĵ°°×Öʱí´ïÇé¿ö¶ÔÓÚÈ«ÃæÁ˽âϸ°ûµÄ¹¦ÄܾßÓÐÖØÒªÒâÒå¡£ÓÉÓÚÑÇϸ°ûµ°°×ÖÊ×éѧÑо¿µÄ×ÅÑÛ µãÔÚµ°°×ÖÊ×éµÄÌض¨³É·Ö£¬¶ø·ÇÕû¸öµ°°×ÖÊ×飬¾ÍÓÐЧµÄÄܹ»ÓÐЧÃÖ²¹Ä¿Ç°µ°°×ÖÊ×éÑо¿·½·¨Ñ§ÉϵIJ»×ã¡£¼´Ò»·½Ãæ¿ÉÒÔΪÎÒÃÇÌṩ¸ü¶àÉúÃü»î¶¯µÄ±¾ÖÊ¡¢Ï¸°û¹¦ÄÜ ÎÉÂҵķÖ×Ó»úÖƵÈÐÅÏ¢¡£ÁíÒ»·½Ãæ¶àÖÖ¼²²¡Ïà¹ØµÄ»ùÒò±í´ï²úÎïÓëijЩÌض¨µÄϸ°ûÆ÷Ïà¹ØÁª£¬Ö±½Ó·ÖÎöÕâЩϸ°ûÆ÷µÄµ°°×Öʱí´ï±ä»¯£¬Äܹ»¸üÈÝÒס¢¸ü¿ìËÙµØÑ°ÕÒµ½ ÓÐÕï¶Ï¡¢ÖÎÁƼÛÖµµÄÐÅÏ¢¡£

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